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Cellular Organization

Investigation 1: Observing Cell Organisms

In Investigation One, you will observe the variables that should be considered when viewing microscopic specimens such as type of staining and section preparation. You will also observe that cells from different organisms often appear similar under a microscope. During this Investigation, you will:

1. View (6) six unknown specimens under the 4x, 10x, 40x, and 100x oil immersion objectives.

2. Observe differences in cell size, shape, organization, and structure.

3. Review the calculation of total magnification:

Total Magnification = eyepiece (10x) X power of objective

Investigation 2: Staining and Sectioning Specimens

In Investigation Two, you will compare specimens that were stained and unstained as well as cross-sectioned and longitudinal sectioned. During this Investigation, you will:

1. Prepare a wet mount slide of your unstained cheek cells and viewed them under the microscope using the 4x, 10x, and 40x objectives.

2. View a prepared slide of stained cheek cells under the microscope using the 4x, 10x, and 40x objectives.

3. Prepare a cross-section of a piece of celery and viewed it under the microscope using the 4x, 10x, and 40x objectives.

4. Prepare a longitudinal section of a piece of celery and viewed it under the microscope using the 4x, 10x, and 40x objectives.

Investigation 3: Structure of Plant and Animal Cells

In Investigation Three, you will compare and contrast the organelle structures in plant and animal cells. During this Investigation, you will:

1. Use the microscope to compare and contrast the cell wall, cell membrane, nucleus, chloroplast, and cytoplasm in the following cell specimens:

  • Onion section
  • Cheek cell
  • Blood smear
  • Elodea leaf
  • Elodea stem
  • Large intestine

Investigation 4: Cell Wall and Cell Membrane

In Investigation Four, you will investigate the permeability of the cell wall and cell membrane. During this Investigation, you will:

1. Prepare (4) four wet mounts of plant tissue with the following salt solutions:

  • tap water
  • 2% salt solution
  • 4% salt solution
  • 8% salt solution

2. Observe the effects of salt solutions on the cell wall, cell membrane, and cytoplasm.

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Cellular Organization